Am J Clin Microbiol Antimicrob | Volume 7, Issue 1 | Research Article | Open Access

Antimicrobial Resistance of Neisseria gonorrhoeae Strains Isolated from Patients with Sexually Transmitted Infection in Dakar, Senegal

Abdou Diop^2*, Babacar Ndiaye², Chantal Mahou Douala-Djemba², Réna Derwiche², Thierno Abdoulaye Diallo² and Abdoulaye Seck^1,2

¹Department of Bacteriology-Virology, Faculty of Medicine, Pharmacy and d’Odonto-stomatology, Cheikh Anta Diop University, Dakar, Senegal ²Medical Biology Laboratory, Pasteur Institute of Dakar, Senegal

^*Correspondance to: Abdou Diop 

Abstract

Background: Neisseria gonorrhoeae is a strictly human pathogenic bacterium responsible for sexually transmitted infections. N. gonorrhoeae is naturally sensitive to many antibiotics, but is characterized by its ability to develop resistance mechanisms. These mechanisms limit treatment options and contributed to high untreated gonorrhea. This aim of this study was to determine antibiotic susceptibilities of N. gonorrhoeae isolates from Dakar. Methods: This retrospective study involved on 90 STI patients from 2020 to 2024. Samples were cultured on Martin Lewis agar and incubated at 36°C ± 1°C in 5% CO2 for 24h to 48h. Identification of N. gonorrhoeae isolates was achieved by Api NH. MICs were determined using E-test method with 0.5 McFarland bacterial suspension. Results: 90 strains of N. gonorrhoeae were isolated from patients and male represented 94.44%. Median age was 45 years and age group most represented was [25-34 years] with 37.78%. The prevalence of resistance was high to tetracycline (85.56%) and ciprofloxacin (86.67%) with MIC90 of 48 μg/mL, and 3 μg/mL respectively. Resistance of isolates to azithromycin was 4.44% (4/90) with MIC ranging from 0.0125 to 2 μg/mL, MIC50 of 0.064 μg/mL, and MIC90 of 0.75 μg/mL. All isolates were sensitive to ceftriaxone, cefixime and gentamycin (100%) with very low MIC50 (0.006 mg/L and 0.016 mg/L) and MIC90 (0.016) for ceftriaxone and cefixime respectively. Conclusion: N. gonorrhoeae resistance to tetracycline and ciprofloxacin was high necessitating revision of the treatment guidelines. However, no resistance to ceftriaxone and cefixime was detected.

Keywords:

Neisseria gonorrhoeae; Antimicrobial resistance; MIC; Senegal

Citation:

Diop A, Ndiaye B, Douala-Djemba CM, Derwiche R, Diallo TA, Seck A. Antimicrobial Resistance of Neisseria gonorrhoeae Strains Isolated from Patients with Sexually Transmitted Infection in Dakar, Senegal. Am J Clin Microbiol Antimicrob. 2025; 7(1): 1067..